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三氯生 1

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Cryo-copolymerization preparation of dextran-hyaluronate based supermacroporous cryogel scaffolds for

Dongjiao ZHOU, Shaochuan SHEN, Junxian YUN, Kejian YAO, Dong-Qiang LIN

《化学科学与工程前沿（英文）》 2012年 第6卷 第3期   页码 339-347 doi: 10.1007/s11705-012-1209-1

摘要： Dextran-hyaluronate (Dex-HA) based supermacroporous cryogel scaffolds for soft tissue engineering were prepared by free radical cryo-copolymerization of aqueous solutions containing the dextran methacrylate (Dex-MA) and hyaluronate methacrylate (HA-MA) at various macromonomer concentrations under the freezing condition. It was observed that the suitable total concentration of macromonomers for the preparation of Dex-HA cryogel scaffold with satisfied properties was 5% (w/w) at the HA-MA concentration of 1% (w/v), which was then used to produce the test scaffold. The obtained cryogel scaffold with 5% (w/w) macromonomer solution had high water permeability (5.1 × 10 m ) and high porosity (92.4%). The pore diameter examined by scanning electron microscopy (SEM) was in a broad range of 50–135 μm with the mean pore diameter of 91 μm. Furthermore, the cryogel scaffold also had good elastic nature with the elastic modulus of 17.47±1.44 kPa. The culture of 3T3-L1 preadipocyte within the scaffold was investigated and observed by SEM. Cells clustered on the pore walls and grew inside the scaffold indicating the Dex-HA cryogel scaffold could be a promising porous biomaterial for applications in tissue engineering.

关键词： cryogel scaffold     tissue engineering     dextran     hyaluronate     3T3-L1 preadipocyte    

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Three-dimensional reconstruction of light microscopy image sections: present and future

null

《医学前沿（英文）》 2015年 第9卷 第1期   页码 30-45 doi: 10.1007/s11684-014-0337-z

摘要：

Three-dimensional (3D) image reconstruction technologies can reveal previously hidden microstructures in human tissue. However, the lack of ideal, non-destructive cross-sectional imaging techniques is still a problem. Despite some drawbacks, histological sectioning remains one of the most powerful methods for accurate high-resolution representation of tissue structures. Computer technologies can produce 3D representations of interesting human tissue and organs that have been serial-sectioned, dyed or stained, imaged, and segmented for 3D visualization. 3D reconstruction also has great potential in the fields of tissue engineering and 3D printing. This article outlines the most common methods for 3D tissue section reconstruction. We describe the most important academic concepts in this field, and provide critical explanations and comparisons. We also note key steps in the reconstruction procedures, and highlight recent progress in the development of new reconstruction methods.

关键词： microtomy     3D imaging     computer-assisted image processing     3D printing     tissue scaffold    

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Current advances for bone regeneration based on tissue engineering strategies

Rui Shi, Yuelong Huang, Chi Ma, Chengai Wu, Wei Tian

《医学前沿（英文）》 2019年 第13卷 第2期   页码 160-188 doi: 10.1007/s11684-018-0629-9

摘要： Bone tissue engineering (BTE) is a rapidly developing strategy for repairing critical-sized bone defects to address the unmet need for bone augmentation and skeletal repair. Effective therapies for bone regeneration primarily require the coordinated combination of innovative scaffolds, seed cells, and biological factors. However, current techniques in bone tissue engineering have not yet reached valid translation into clinical applications because of several limitations, such as weaker osteogenic differentiation, inadequate vascularization of scaffolds, and inefficient growth factor delivery. Therefore, further standardized protocols and innovative measures are required to overcome these shortcomings and facilitate the clinical application of these techniques to enhance bone regeneration. Given the deficiency of comprehensive studies in the development in BTE, our review systematically introduces the new types of biomimetic and bifunctional scaffolds. We describe the cell sources, biology of seed cells, growth factors, vascular development, and the interactions of relevant molecules. Furthermore, we discuss the challenges and perspectives that may propel the direction of future clinical delivery in bone regeneration.

关键词： bone tissue engineering     stem cell     bone scaffold     growth factor     bone regeneration    

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Current progress on scaffolds of tissue engineering heart valves

DONG Nianguo, SHI Jiawei, CHEN Si, HONG Hao, HU Ping

《医学前沿（英文）》 2008年 第2卷 第3期   页码 229-234 doi: 10.1007/s11684-008-0043-9

摘要： Tissue engineering heart valves (TEHV) may be the most promising valve substitute, but the study has been relatively stagnant in the recent five years due to the special position, function and mechanical property of heart valves. It is one of the key factors to select an ideal scaffold material in the construction of TEHV. And this article will briefly review the current research and progress on the scaffolds of TEHV, especially based on Chinese works.

关键词： function     scaffold     promising     research     engineering    

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New branched benign compounds including double antibiotic scaffolds: synthesis, simulation and adsorption for anticorrosion effect on mild steel

《化学科学与工程前沿（英文）》 2023年 第17卷 第2期   页码 167-182 doi: 10.1007/s11705-022-2199-2

摘要： In this study, two novel environmental benign double antibiotic norfloxacin or ciprofloxacin scaffolds included branched molecules were prepared by multi-step routes and purified by simple performance, which were used as the target compounds (TCs). Meanwhile, a single norfloxacin or ciprofloxacin skeleton based molecules were synthesized as the reference compounds (RCs). The molecular geometry optimization and material simulation computation revealed that TCs presented smaller HOMO-LUMO energy gaps and larger binding energy levels on mild steel surface than RCs. The chemical adsorption of TCs on steel surface was confirmed by X-ray photoelectron spectroscopy, which could be processed by TCs chelation with iron ions. It was shown that TCs could be self-adsorbed on steel surface, which was demonstrated by atomic force microscopy and scanning electron microscopy. The anticorrosion of the studied compounds for mild steel in HCl solution was investigated by electrochemistry analysis. The results suggested that the anticorrosion efficiency could reach 95.86% (TC1) and 97.05% (TC2) at 0.050 mmol·L−1 based on electrochemical impedance spectroscopy, which were much better than RCs (RC1, 69.23%; RC2, 74.16%). The adsorption isotherms of TCs on steel were further fitted, and a deep insight on adsorption was discussed.

关键词： branched compounds     floxacin scaffold     mild steel     anticorrosion     environmentally benign    

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纤维基质——乳腺癌检测和治疗的新途径 Review

Rasha Rezk,  Raquel Marín-García,  Annica K.B.Gad

《工程（英文）》 2021年 第7卷 第10期   页码 1375-1380 doi: 10.1016/j.eng.2021.04.024

摘要：

乳腺癌的特点是肿瘤细胞周围的蛋白纤维大量增加。这些纤维会提高组织的机械硬度，人们一直以来都是利用这一点并通过手动触诊来诊断肿瘤。最近的生物工程研究开发了新型生物材料，这些材料模拟了肿瘤微环境的力学特性和结构特性，可以用来了解这些特性如何调节乳腺癌的发展和扩散。本文概述了乳腺癌组织的力学特性与正常乳腺组织及非癌性病变组织的力学特性之间的差异，描述了生物材料模型是如何用于了解细胞外环境的硬度和黏度调节细胞迁移和乳腺癌转移的。此外，本文还强调了对生物材料模型的需求，这些模型可独立分析肿瘤微环境的单个和多个力学特性并利用肿瘤内不同区域的细胞，同时为进行乳腺癌转移新型机械疗法的开发提供了指导。

关键词： 乳腺癌     组织硬度     癌症转移     细胞迁移     生物工程支架     黏度    

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Hemocompatible polyurethane/gelatin-heparin nanofibrous scaffolds formed by a bi-layer electrospinning technique as potential artificial blood vessels

Heyun WANG, Yakai FENG, Marc BEHL, Andreas LENDLEIN, Haiyang ZHAO, Ruofang XIAO, Jian LU, Li ZHANG, Jintang GUO

《化学科学与工程前沿（英文）》 2011年 第5卷 第3期   页码 392-400 doi: 10.1007/s11705-011-1202-0

摘要： In this paper, a scaffold, which mimics the morphology and mechanical properties of a native blood vessel is reported. The scaffold was prepared by sequential bi-layer electrospinning on a rotating mandrel-type collector. The tubular scaffolds (inner diameter 4 mm, length 3 cm) are composed of a polyurethane (PU) fibrous outer-layer and a gelatin-heparin fibrous inner-layer. They were fabricated by electrospinning technology, which enables control of the composition, structure, and mechanical properties of the scaffolds. The microstructure, fiber morphology and mechanical properties of the scaffolds were examined by means of scanning electron microscopy (SEM) and tensile tests. The PU/gelatin-heparin tubular scaffolds have a porous structure. The scaffolds achieved a breaking strength (3.7±0.13 MPa) and an elongation at break (110±8%) that are appropriate for artificial blood vessels. When the scaffolds were immersed in water for 1 h, the breaking strength decreased slightly to 2.2±0.3 MPa, but the elongation at break increased to 145±21%. In platelet adhesion tests the gelatin-heparin fibrous scaffolds showed a significant suppression of platelet adhesion. Heparin was released from the scaffolds at a fairly uniform rate during the period of 2 day to 9 day. The scaffolds are expected to mimic the complex matrix structure of native arteries, and to have good biocompatibility as an artificial blood vessel owing to the heparin release.

关键词： electrospinning     artificial blood vessels     scaffold     polyurethane     gelatin     nanofiber     hemocompatibility    

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面向医学应用的3D 打印技术现状综述 Review

严倩,董汉华,苏瑾,韩建华,宋波,魏青松, 史玉升

《工程（英文）》 2018年 第4卷 第5期   页码 729-742 doi: 10.1016/j.eng.2018.07.021

摘要：

器官移植供体短缺一直是世界各国医学界面临的重大难题之一。传统方法不可避免地存在并发症、二次损伤和供体来源有限等潜在问题。3D 打印技术具有解决目前这些问题的潜力，可用于快速制造个性化组织工程支架、细胞原位修复组织缺陷，甚至直接打印组织和器官。打印的植入体和器官不仅与患者的受损组织完美匹配，而且能够调控材料微结构和细胞排列，促进细胞生长和分化。因此，这类植入体有望实现组织修复，甚至最终解决供体短缺问题。本文总结了3D 打印在医学领域相关研究和最新进展的四个层次，介绍了不同类型的生物医用材料，并讨论了医学应用中3D 打印所需材料和构建体外细胞质基质（ECM）存在的问题和未来的发展方向。

关键词： 3D 打印     植入体     支架     生物医学材料    

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Effect of incorporating Elaeagnus angustifolia extract in PCL-PEG-PCL nanofibers for bone tissue engineering

Vahideh R. Hokmabad, Soodabeh Davaran, Marziyeh Aghazadeh, Effat Alizadeh, Roya Salehi, Ali Ramazani

《化学科学与工程前沿（英文）》 2019年 第13卷 第1期   页码 108-119 doi: 10.1007/s11705-018-1742-7

摘要：

Plants have been used for medicinal purposes for thousands of years but they are still finding new uses in modern times. For example, Elaeagnus angustifolia (EA) is a medicinal herb with antinociceptive, anti-inflammatory, antibacterial and antioxidant properties and it is widely used in the treatment of rheumatoid arthritis and osteoarthritis. EA extract was loaded onto poly(ϵ-caprolactone)-poly(ethylene glycol)-poly(ϵ-caprolactone) (PCL-PEG-PCL/EA) nanofibers and their potential applications for bone tissue engineering were studied. The morphology and chemical properties of the fibers were evaluated using Fourier transform infrared spectroscopy, field emission scanning electron microscopy, contact angle measurements and mechanical tests. All the samples had bead-free morphologies with average diameters ranging from 100 to 200 nm. The response of human cells to the PCL-PEG-PCL/EA nanofibers was evaluated using human dental pulp stem cells (hDPSCs). The hDPSCs had better adhesion and proliferation capacity on the EA loaded nanofibers than on the pristine PCL-PEG-PCL nanofibers. An alizarin red S assay and the alkaline phosphatase activity confirmed that the nanofibrous scaffolds induced osteoblastic performance in the hDPSCs. The quantitative real time polymerase chain reaction results confirmed that the EA loaded nanofibrous scaffolds had significantly upregulated gene expression correlating to osteogenic differentiation. These results suggest that PCL-PEG-PCL/EA nanofibers might have potential applications for bone tissue engineering.

关键词： Elaeagnus angustifolia     scaffold     electrospinning     human dental pulp stem cell     tissue engineering    

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关于3D打印技术在医学模具以及再生组织和器官方面的应用综述

Kan Wang, Chia-Che Ho, Chuck Zhang, Ben Wang

《工程（英文）》 2017年 第3卷 第5期   页码 653-662 doi: 10.1016/J.ENG.2017.05.013

摘要：

医学模型或模具已广泛应用于医学培训和医患互动领域，同时也日益应用于手术规划、医学计算模型、算法核实和验证以及医疗器械的开发等方面。这种新应用需要高保真度、患者特异性及模拟组织结构的医学模具，其不仅能够模拟人体器官的几何结构，而且还具有器官的功能。随着三维（3D）打印和3D 生物打印技术的快速发展，许多研究人员已经开始使用增材制造技术来生产具有多种功能的医学模具。本文综述了3D 打印和3D 生物打印技术在制作功能性医学模具和生物结构方面的应用。特别讨论了3D 打印功能性医学模具（即组织模拟医学模具、放射性医学模具和生理医学模具）及被用于再生组织和器官的3D 生物打印模具的制备（即混合模式支架材料、可转换支架和集成传感器）工艺、发展现状以及未来发展趋势。

关键词： 3D打印     3D生物打印     医学模具     再生组织/器官     支架    

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三维仿生支架平台的刚度和层尺寸对癌细胞分离的影响 Article

张维冠, 刘子玉, 彭慧芝

《工程（英文）》 2021年 第7卷 第10期   页码 1426-1435 doi: 10.1016/j.eng.2020.09.010

摘要：

癌细胞分离是癌症诊断和治疗的理想手段。除了生化方法，工程化平台是根据癌细胞响应周围微环境物理变化的独特特性将癌细胞与正常细胞分离的有效选择。本研究根据精确控制的设计参数（包括刚度、层数和结构布局），开发了三维（3D）仿生支架平台，用于分离鼻咽癌（NPC43）细胞与永生化鼻咽上皮（NP460）细胞。支架平台上NPC43细胞和NP460细胞的迁移特征表明，NPC43细胞可以挤进10 µm宽、15 µm深的沟槽，而NP460细胞不能。迁移行为的不同主要是由细胞与周围微环境的交互作用不同所导致。NPC43细胞具有丝状伪足样突触，而NP460细胞呈片状形态。使用这些3D仿生平台进行研究发现，在较硬的双层支架平台[顶层为40/10 μm沟脊/沟槽（R/T）栅格，底层为20/10 μm R/T网格]上，NPC43细胞与NP460细胞的分离效率可达89%。此外，通过添加活性条件培养基（ACM）可使细胞具有更高的运动性和变形性，从而将分离效率进一步提高到93%。这些结果表明，研究人员可以利用设计适当的仿生工程化平台分离癌细胞和正常细胞，从而辅助实现癌症诊断和治疗。

关键词： 细胞分离     细胞迁移     鼻咽癌（NPC）     3D支架平台    

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一步法绿色制备具有持续抗菌性能的医用支架 Article

黄榕康, 胡民辉, 梁伟文, 郑娟娟, 杜杨, 林燕欢, 王怀明, 过文泰, 曾展涛, 李创坤, 利民, 王辉, 张兴才

《工程（英文）》 2021年 第7卷 第3期   页码 326-333 doi: 10.1016/j.eng.2020.08.022

摘要：

聚丙烯（polypropylene, PP）支架是目前最常用的生物医用支架，在外科手术使用中存在黏连、感染、异物反应等缺点。本文报道了一种简易一步法，用于制备新型三氯生聚多巴胺聚丙烯（TPP）复合支架，从而有效地提高了PP支架的生物相容性和持续抗菌性。通过一步法绿色制造，具有广谱抗菌效果的三氯生在聚多巴胺形成过程中，在PP支架表面可以有效地与多巴胺发生相互作用。三氯生可从具有生物相容性的聚多巴胺表面涂层持续释放。利用三氯生（浓度为8 mg∙mL−1，即TPP-8）制备的5 mm × 5 mm的支架具备持续抗菌性，对最大菌液量为2 mL的大肠杆菌（E. coli）和5 mL的金黄色葡萄球菌（S. aureus）的持续抗菌时间超过15 d。本研究为长效抗菌医用生物材料的绿色制备提供了一个新的方向。

关键词： 绿色化学     聚丙烯支架     聚多巴胺     三氯生     抗菌    

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标题 作者 时间 类型 操作

Cryo-copolymerization preparation of dextran-hyaluronate based supermacroporous cryogel scaffolds for

Dongjiao ZHOU, Shaochuan SHEN, Junxian YUN, Kejian YAO, Dong-Qiang LIN

期刊论文

Three-dimensional reconstruction of light microscopy image sections: present and future

null

期刊论文

Current advances for bone regeneration based on tissue engineering strategies

Rui Shi, Yuelong Huang, Chi Ma, Chengai Wu, Wei Tian

期刊论文

Current progress on scaffolds of tissue engineering heart valves

DONG Nianguo, SHI Jiawei, CHEN Si, HONG Hao, HU Ping

期刊论文

New branched benign compounds including double antibiotic scaffolds: synthesis, simulation and adsorption for anticorrosion effect on mild steel

期刊论文

纤维基质——乳腺癌检测和治疗的新途径

Rasha Rezk,  Raquel Marín-García,  Annica K.B.Gad

期刊论文

Hemocompatible polyurethane/gelatin-heparin nanofibrous scaffolds formed by a bi-layer electrospinning technique as potential artificial blood vessels

Heyun WANG, Yakai FENG, Marc BEHL, Andreas LENDLEIN, Haiyang ZHAO, Ruofang XIAO, Jian LU, Li ZHANG, Jintang GUO

期刊论文

面向医学应用的3D 打印技术现状综述

严倩,董汉华,苏瑾,韩建华,宋波,魏青松, 史玉升

期刊论文

Effect of incorporating Elaeagnus angustifolia extract in PCL-PEG-PCL nanofibers for bone tissue engineering

Vahideh R. Hokmabad, Soodabeh Davaran, Marziyeh Aghazadeh, Effat Alizadeh, Roya Salehi, Ali Ramazani

期刊论文

关于3D打印技术在医学模具以及再生组织和器官方面的应用综述

Kan Wang, Chia-Che Ho, Chuck Zhang, Ben Wang

期刊论文

三维仿生支架平台的刚度和层尺寸对癌细胞分离的影响

张维冠, 刘子玉, 彭慧芝

期刊论文

一步法绿色制备具有持续抗菌性能的医用支架

黄榕康, 胡民辉, 梁伟文, 郑娟娟, 杜杨, 林燕欢, 王怀明, 过文泰, 曾展涛, 李创坤, 利民, 王辉, 张兴才

期刊论文